Nos produits de laboratoire

ANTICORPS PRIMAIRE: ORIGENE PRIMAIRE / SECONDAIRE: AUTRES SECONDAIRE: JACKSON IMMUNORESEARCH LÉGUMES: AGRISERA VÉTÉRINAIRE BIOLOGIE MOLÉCULAIRE ET CELLULAIRE DÉSINFECTANTS POUR HOTTES ET SALLES DE BAINS KIT D’EXTRACTION KIT PCR / TEMPS RÉEL ET qPCR RÉACTIFS SÉPARATION BIOMAGNÉTIQUE CYTOGÉNÉTIQUE SONDES POUR POISSONS PADS POUR POISSON DMSO ENZYMES HPLC, LC-MS / MS HPLC LC-MS / MS IMMUNOESSAIS ARRAY CLIA / TURBIDIMÉTRIE COLORIMÉTRIQUE ELISA ELISPOT FIXATION DU COMPLEMENT / IF / LIA FLUOROSPOT TEST RAPIDE PRODUITS SUPPLÉMENTAIRES LIGNES MOBILES PROTÉINES ET […]

Polymerase Chain Reaction (PCR) Protocol

Primer can be stored in the refrigerator 4 degrees if not suspended in water when resupending in water, using sterile water when uncapping the tube, do it gently to prevent the spread of lyophilized primer add enough water to have 100pmol / uL OR 1UG / uL The primary stock dilute to 10 pmol / uL OR 100ng / uL to obtain a working solution. Polymerase Chain Reaction (PCR) Protocol Include a negative control DNA template is […]

Solubilizing Membrane Proteins with Triton X-100 Protocol

10mm Tris, 2mm EDTA (TE buffer) 2% Triton in PBS (make a stock solution of 20% Triton X-100 by weighing 2 g of Triton X-100, adding up to 10 mL PBS, rocking or gentle mixing overnight; a stock solution store in the refrigerator at 4 degrees C). Solubilizing Membrane Proteins with Triton X-100 Protocol 1. resuspend cells in TE buffer 2. Wash 2x in TE (rotating in 40,000 x g, 8 minutes each time) 3. To 2% […]

Designing Primers – A Checklist

Do annealing temperature of each primer pair PCR products corresponding to the same? Whether primary has annealing segment? segment tags? restriction enzyme sites? Extra bases to help cut restriction sites? Designing Primers – A Checklist The primary is in the correct orientation (if necessary, it in reverse complement? If necessary, is extra base [d] in a complementary way around?) Annealing temperature is at least 50 degrees Celsius?

Cell Fractionation ProtocolCell Fractionation Protocol

Make sure the digestive really digest Cell Fractionation Protocol 1. It is easier to cut from the vector that has an insert of one who doesnt 2. As a control vector digested with an enzyme only (if one of the cutting enzyme does, that the line will run probably slightly lower as supercoiled form?) 3. To double digest, digesting the first with less efficient enzyme to prevent the generation of oligonucleotide overhang where less efficient enzyme wont […]

Cell Fractionation Protocol

10mm Tris, 2mm EDTA, pH 7.5 For 25 mL, the use of (a) 250uL 1M Tris + (b) 500uL of 0.1 M EDTA. Cell Fractionation Protocol sugar with 20mm Tris buffer, 0.5 M EDTA, and 0.1 M NaCl To 2 mL, the use of (a) 40uL 1M Tris + (b) 10ul of 0.1 M EDTA + (c) 200ul 1M NaCl + (D) 1.2g sucrose + (e) water 1.75mL Also make sugar without sugar 20ml buffer (to thin […]

DNA Ligation Protocol

DNA Ligation Protocol 1. The vector and insert run on an agarose gel along with a DNA ladder 2. quantitate the amount of vector and insert are present: for 6UL of 1 / 16x diluted DNA ladder, 375ng of DNA present in 1650 the band (Gibco) estimate the number of vector and insert DNA relative to the 1650 Band Also take into account that the DNA twice during 1650 bands to two times brighter when the DNA […]

SARS-CoV-2 Antigen Rapid Test Kit (Colloidal Gold)

SARS-CoV-2 Antigen Fast Check Package (Colloidal Gold) 【PRODUCT NAME】 SARS-CoV-2 Antigen Fast Check Package (Colloidal Gold) 【PACKAGE AND SPECIFICATION】 20 Exams/field (1Test/bag ×20 Baggage) ,40 Exams/field (1Test/bag ×40 Baggage) 【INTENDED USE】 For in vitro qualitative detection of SARS-CoV-2 nucleocapsid antigen in nasal (NS) swab specimens straight from people who’re suspected of COVID-19 by their healthcare supplier throughout the first 5 days after onset of signs. This take a look at is barely offered to be used by […]

LIGAND BINDING ASSAY using coated monoclonal antibodies

SMALL MOLECULES / PEPTIDES ANALYTICAL / LIGAND BINDING ASSAYS – ELISA PK – PEPTIDE DRUG ASSAYS KBI5001 KRIBIOLISA™ Angiotensin II ELISA ELISA 96 wells KBI5002 KRIBIOLISA™ BNP 32 (Human) (Natrecor) ELISA ELISA 96 wells KBI5003 KRIBIOLISA™ Buserelin (Suprefact) ELISA 96 wells KBI5004 KRIBIOLISA™ Calcitonin (Salmon) (Miacalcic) ELISA ELISA 96 wells KBI5005 KRIBIOLISA™ Chrysalin (PT508) ELISA ELISA 96 wells KBI5006 KRIBIOLISA™ D-Arg8-vasopressin ELISA ELISA 96 wells KBI5007 KRIBIOLISA™ Davalintide ELISA ELISA 96 wells KBI5008 KRIBIOLISA™ Degarelix (Firmagon) ELISA […]

Ligand Binding Assays – ELISA

Ligand Binding Assays – ELISA PHARMACOKINETIC ASSAYS For pharmacokinetic study of mAbs, FDA and EMA have issued specific guidance for the validation of pharmacokinetic methods that require full method validation for assays that are used to measure monoclonal antibody concentrations in biological matrices at both preclinical and clinical phases. Krishgen Biosystem’s collection comprises a number for mAbs ELISA kit for pharmacokinetic studies to meet the regulatory requirements. KRIBIOLISA Gentaur Elisa Kits are a dedicated range designed to […]