DNA Ligation Protocol

DNA Ligation Protocol 1. The vector and insert run on an agarose gel along with a DNA ladder 2. quantitate the amount of vector and insert are present: for 6UL of 1 / 16x diluted DNA ladder, 375ng of DNA present in 1650 the band (Gibco) estimate the number of vector and insert DNA relative to the 1650 Band Also take into account that the DNA twice during 1650 bands to two times brighter when the DNA […]