Cell Fractionation ProtocolCell Fractionation Protocol

Make sure the digestive really digest Cell Fractionation Protocol 1. It is easier to cut from the vector that has an insert of one who doesnt 2. As a control vector digested with an enzyme only (if one of the cutting enzyme does, that the line will run probably slightly lower as supercoiled form?) 3. To double digest, digesting the first with less efficient enzyme to prevent the generation of oligonucleotide overhang where less efficient enzyme wont […]