Solubilizing Membrane Proteins with Triton X-100 Protocol

10mm Tris, 2mm EDTA (TE buffer) 2% Triton in PBS (make a stock solution of 20% Triton X-100 by weighing 2 g of Triton X-100, adding up to 10 mL PBS, rocking or gentle mixing overnight; a stock solution store in the refrigerator at 4 degrees C). Solubilizing Membrane Proteins with Triton X-100 Protocol 1. resuspend cells in TE buffer 2. Wash 2x in TE (rotating in 40,000 x g, 8 minutes each time) 3. To 2% […]