Solubilizing Membrane Proteins with Triton X-100 Protocol

10mm Tris, 2mm EDTA (TE buffer)

2% Triton in PBS (make a stock solution of 20% Triton X-100 by weighing 2 g of Triton X-100, adding up to 10 mL PBS, rocking or gentle mixing overnight; a stock solution store in the refrigerator at 4 degrees C).

Solubilizing Membrane Proteins with Triton X-100 Protocol

1. resuspend cells in TE buffer
2. Wash 2x in TE (rotating in 40,000 x g, 8 minutes each time)
3. To 2% Triton in PBS while stirring, add dropwise cells were suspended in ~ 1mL TE
4. dissolve for 30 minutes in a cold room after the cells added dropwise
5. Play at 100,000 x g, 10 min
6. Take the supernatant